Coding

Part:BBa_K1945004:Design

Designed by: Sharon Lian   Group: iGEM16_CSU_Fort_Collins   (2016-10-21)


N-terminal fragment of split T7 RNA polymerase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When paired with the N-terminal sequence of T7 RNA polymerase, they can be used to create an AND gate.

Source

From bacteriophage T7.

References

This split T7 system for building an AND gate was taken from Shis, D. L., & Bennett, M. R. (2013). Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proceedings of the National Academy of Sciences, 110(13), 5028-5033. doi:10.1073/pnas.1220157110